Arab Private University for Science and Technology
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Faculty of Pharmacy / Teachers
![Salim Al-Hafyan]()
Dr Salim Al-Hafyan
Faculty Member
Certifications
- PhD in Biochemistry - University of Tarbiat Modares - Iran 2023.
- Master of Biology- Biochemistry - University of Ferdowsi Mashhad - Iran 2018.
- Bachelor of Chemistry Department- Biochemistry - University of Aleppo - Syria 2012.
Languages
Skills
- Researcher at Biochemistry and Biophysics Research lab Quantification of Vascular Endothelial Growth Factor (VEGF) by nanoparticle Labeled Specific Phage - Tarbiat Modares university \ Iran 2023.
Tasks and Achievements:- Production and purification of QDs, Production of Anti-VEGF Phage Displaying VHH and The direct conjugation of TGA- CdTe QDs to the amino groups of phages (PVIII) was achieved through amine-carboxyl acid coupling using EDC/Sulfo-NHS as a chemical cross-linker.
- Characterization and Spectral Measurements (such as UV Spectroscopy, Fluorescence spectroscopy, DLC Fluorescence plate reader, Phage Display-Mediated Immuno-Fluorescence (PD-IFL) Assay for VEGF Sensing). Purification and Expression of Recombinant VEGF8–109 in E. coli Strain, SHuffle.
- Calculation of (LOD, LOQ), Validation Assay of VEGF Biosensor.
- Researcher at Biochemistry and Biophysics Research lab Study of the ameliorative effect of betanie on oxidative stress induced by arsenic Poisoning in liver and kidney of rats - Ferdowsi Mashhad - Iran 2018
Tasks and Achievements- investigate the ameliorative effects of betaine versus arsenite-induced alterations of some biochemical parameters indicative of oxidative stress and infliction of the liver and kidney.
- Effects of betaine and As on GPx activity, GSH, CAT, MDA and SOD in rat liver and kidney.
- The Sixth Scientific Conference of Syrian Researchers at Home and Abroad 2024 - July 2024.
- Molecular Cloning, Cell-Surface Displayed Expression of VHH Against VEGF Expressed in E. Coli by Ice Nucleation Protein (INP) - June 2024.
- A member of the Scientific and Executive team of the 22 nd National and 10 th international Congress on Biology - September 2022.
- Participant in the course on Ethical Experiments and Procedures Involving Animals - March 2017.
- Participant in the course on Ethical Experiments and Procedures Involving Humman - December 2021.
- Participating member in the Syrian Cancer Society of Homs - January 2024.
- Associate member of the Syrian Chemical Society - January 2024.
- Environmentally friendly activities at the University of Aleppo, Al-Sham Special, Ferdowsi Mashhad and Tarbiat Modares university
Other Information
- Wet Laboratory Skills:
- PCR-Agarose.
- Gel Electrophoresis
- SDS-PAGE.
- Cell Culture.
- Bradford Assay.
- Transformation.
- Western Blotting.
- Fluorescence spectroscopy.
- Magnetite nanoparticle for improving protein stability
- Chemiluminescence
- Protein Purification
- Proteins Refolding Techniques.
- PCR
- RT-PCR
- Dilution.
- Plasmid Extraction.
- Ligand Binding Assays.
- NMR(Theory).
- Molecular Cloning UV.
- Spectroscopy.
- ELISA.
- Fluorescence-ELISA.
- Protein Production.
- DNA Extraction.
- CRISPAR-CAS9.
- Chimeric Protein.
- Enzyme Kinetic Assay.
- Circular Dichroism.
- DSC.
- DLC.
- CD.
- HPLC chromatography.
- TLC.
- Synthesis of TGA.
- Capped CdTe QDs.
- Dry lab Skills:
Molecular Docking - Molecular Dynamic simulation (in progress) - Homology Modelling Virtual Drug Screening - Virtual Cloning - Primer Design. - Computer Skills:
- Biology Databases: NCBI, ExPASy, Swiss-Prot, UniProt, PDB, EMBL-EBI, OMIM, Ensemble, GenBank,Nucleotide, Human Protein Atlas, MitoCarta2.0, KEGG, Genome, DoE, UCSC, New England Biolabs, REBASE, Takara Bio, HMDB, Drug Bank, ZINC.
- Servers: Primer3Plus, Dotmatcher, IDT, EMBOSS Seqret, Disulfide by Design, EMBOSS Needle, BLAST, dezyme, ROSIE.
