A pragmatic, economical, precise, straightforward to execute, and selective method for separation and determination of Aspartame and Acesulfame-k concentrations in non-alcoholic beverages and soft drinks.The separation by HPLC was implemented using a Welchrom C18 column (4.6 x 250 mm, 5µm) at 30°C temperature and a SHIMADZU UV-photo diode array detector at 217, 226 nm for Aspartame and Acesulfame-k respectively.The mobile phase composition consisted of potassium dihydrogen phosphate pH= 4.5 and acetonitrile (80:20 v/v) with a flow rate of 1mL/min and an injection volume of 10 µL.The retention time of Acesulfame-k and Aspartame were 2.94, 6.51 respectively. The analysis time was less than 10 minutes. This method demonstrated appropriate results of linearity, precision, and recovery. It was applied efficiently to analyze Aspartame and Acesulfame-k.The calibration curve was linear with R2 >0.999. The precision values of percentage relative standard deviation were less than 2 %RSD
Bulletin of Pharmaceutical Science - Assiut.
2022.
A Validated HPLC Method for Separation and Determination Aspartame and Acesulfame-k in Food Products