The purpose of this article is how to determine simultaneously the content of some Cephalosporins: Cefaclor, Cefalexin, Cefadroxil in pure and pharmaceutical formulations by high performance liquid chromatography. Analysis was conducted on a C18- Column (250 x 4.6mm, 5μm, 10A:), and used mobile phase consisting of (Triethylamine: Methanol: Acetonitrile: water) with ratios (2:10:20:68) v/v% respectively, and flow rate for mobile phase 1ml/min. An ultraviolet detector was used at wavelength of 265nm.
Results were linear in the range of (0.1-80) μg.mL-1.The correlation coefficients were ( 0.9995, 0.9997. 0.9995) Cefaclor, Cefalexin, and Cefadroxil for respectively, the limits of detection were (0.87, 0.67 ,0.86) μg.mL-1 so forth.
Characterized method applied good accuracy, through RSD% less than 2% values, excellent precision, which manifested itself recovery % within the allowable area according to the (USP) values. This method can be used in analysis of quality control laboratories in pharmaceutical research and drug control.
Homs University Journal.
2015.