An accurate, rapid and sensitive RP-HPLC method has been developed to determine HMF concentrations in pure and adulterated Syrian honey samples by beefeeding or with invert syrup.
The separation process was developed under optimized chromatographic conditions using C18 column and a mobile phase that consists of (methanol: water with acetic acid 3%) (15:85) the flow rate was 0.9 ml/min and UV detection at 284 nm; the retention time for the developed method was 7.9 minute, the linearity, repeatability, accuracy, and precision of the method were determined. The detection limit and quantification limit were 0.07 mg/kg and 0.22 mg/kg, respectively and R2 was 0.9999.
The developed method was applied to HMF concentrations in Syrian honey samples, The study showed that HMF concentrations were (2.2-26) mg/kg in newly collected samples and (7.2-106.2) mg/kg in samples stored for one year.
As a result of this study, we notice that HMF concentrations were significantly higher in the adulterated honey samples and exceeded the international standards after one year of storage. On the other hand, HMF concentrations in pure samples were within the limits of international specification and Syrian standards in newly collected samples and those stored for one year.
Research Journal of Aleppo University.
2019.
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