black seeds known as nigella sativa contains thymoquinone which was used in many general medicins as an active antibiotic in general and oral health. so, we aimed in our research to study the effect of nigella sativa on dental caries bacteria using filter paper method on mueller hinton media (Kirby baure method) , and compare its effect with 9 other antibiotics.
Methods :
25 dental caries specimens were collected from class 1 caries on 25 upper first molars . Mani tingesten carbide burs were used for caries curettage (one bur for every tooth ).
and by using the filter paper method , bacterial culture were first grown in nutrient borth at 37 for 18-24 h incubated. then after using blood agar & EMB agar for germs cultivation , the respective bacteria were spread on to the mueller hinton agar plates using sterile swap. filter paper method were used to investigate the antibacterial activity of nigella sativa and comparing the diameters of circle around nigella sativa paper with diameters around 10 studied antibiotics .
Results:
the results showed inhibition zone around the nigella sativa paper measured about 1- 1.9cm, antibiotics zone was 1.1-2.8 cm.
Conclusion:
our study showed antibacterial activity of nigella sativa against dental caries germs.
Damascus University Journal.
2022.
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