A High Performance liquid chromatographic method was developed for the determination of Cephalosporins like as: Cefaclor monohydrate, Cefalexine monohydrate, Cefadroxil monohydrate in pharmaceutical dosage forms. Isocratic separation was performed on an Enable C18 column (250 mm × 4.6 mm i.d., 5.0 μm) using Triethylamine: Methanol: Acetonitrile: Ultra Pure Water (2: 10: 20: 68 v/v%) as the mobile phase at a flow rate of 1.0 ml/min. The PDA detection wavelength was set at 265nm. The linearity was observed over a concentration range of 0.1–80 μg/ml for HPLC method (correlation coefficient=0.999). The developed methods were validated according to ICH guidelines. The relative standard deviation values for the method precision studies were < 2%, and the accuracy was > 99%. The developed method was used successfully for the determination of Cefaclor, Cephalexin, Cefadroxil in Capsule, Tablet and dry syrup formulations.
Chemistry and Materials Research.
2015.
Determination of Some Cephalosporins in Pharmaceutical Dosage Forms by RP-HPLC Method